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Application of the mixed model method requires data sets in which all individuals are measured for genome-wide genotypes and the environmental risk factor. The mixed linear model method requires large samples measured for both individual level genetic and environmental measures (based on power considerations (e.Dudbridge, 2013; Visscher et al. Tarantool-lts 15537g1687c02-1 binary overridden individual studies are generally too small, researchers are likely to combine data from several studies into one study.

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