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TrackMate is used for: We present TrackMate, an open source Fiji plugin for the automated, semi-automated, and manual tracking of single-particles. It offers a versatile tracking bbinary com 602016 modular solution that works out of the box for end users, through a simple and intuitive user interface.

It is also easily scriptable and adaptable, operating equally well on 1D over time, 2D over time, 3D over time, or tracking bbinary com 602016 single and multi-channel image variants. TrackMate provides several visualization and analysis tools that aid in assessing the relevance of results. The utility of TrackMate is further enhanced through its ability to be readily customized to meet specific tracking problems.

TrackMate is an extensible platform where developers can easily write their own detection, particle linking, visualization or analysis algorithms within the TrackMate environment. This evolving framework provides researchers with the opportunity to quickly develop and optimize new algorithms based on existing TrackMate modules without the need of having to write de novo user interfaces, including visualization, analysis and exporting tracking bbinary com 602016.

The current capabilities of TrackMate are presented in the context of three different biological problems. First, we perform Caenorhabditis-elegans lineage analysis to assess how light-induced damage during imaging impairs its early development. Our TrackMate-based lineage analysis indicates the lack of a cell-specific light-sensitive mechanism. Finally, we validate the use of TrackMate for quantitative lifetime analysis of clathrin-mediated endocytosis in plant cells.

Download high-res image KB Download full-size image. Methods Volume15 FebruaryPages Hoopes c Gregory D. Reynolds c Emmanuel Laplantine d Sebastian Y. Bednarek c Spencer L. Shorte a Kevin W. Under a Creative Commons license. Abstract We present TrackMate, an open source Fiji plugin for the tracking bbinary com 602016, semi-automated, and manual tracking of single-particles.

Graphical abstract Download high-res image KB Download full-size image. Recommended articles Citing articles 0. Published by Elsevier Inc.

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